Lysine β311 of Protein Geranylgeranyltransferase Type I Partially Replaces Magnesium
نویسندگان
چکیده
منابع مشابه
Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I
More than 100 proteins necessary for eukaryotic cell growth, differentiation, and morphology require posttranslational modification by the covalent attachment of an isoprenoid lipid (prenylation). Prenylated proteins include members of the Ras, Rab, and Rho families, lamins, CENPE and CENPF, and the g subunit of many small heterotrimeric G proteins. This modification is catalyzed by the protein...
متن کاملSmall-molecule inhibitors of protein geranylgeranyltransferase type I.
Background In recent years, organocatalysis has emerged as a powerful technology, allowing synthetic chemists to perform various types of reactions. The catalytic activity of the process relies on low-molecular-weight organic molecules and does not require involvement of transition or main group metals. The organocatalytic method has become a very attractive strategy, particularly in the synthe...
متن کاملProtein geranylgeranyltransferase-I of Trypanosoma cruzi.
Protein geranylgeranyltransferase type I (PGGT-I) and protein farnesyltransferase (PFT) occur in many eukaryotic cells. Both consist of two subunits, the common alpha subunit and a distinct beta subunit. In the gene database of protozoa Trypanosoma cruzi, the causative agent of Chagas' disease, a putative protein that consists of 401 amino acids with approximately 20% amino acid sequence identi...
متن کاملInhibitors of protein geranylgeranyltransferase I and Rab geranylgeranyltransferase identified from a library of allenoate-derived compounds.
Protein geranylgeranylation is critical for the function of a number of proteins such as RhoA, Rac, and Rab. Protein geranylgeranyltransferase I (GGTase-I) and Rab geranylgeranyltransferase (RabGGTase) catalyze these modifications. In this work, we first describe the identification and characterization of small molecule inhibitors of GGTase-I (GGTI) with two novel scaffolds from a library consi...
متن کاملCDC43 and RAM2 encode the polypeptide subunits of a yeast type I protein geranylgeranyltransferase.
The question regarding the identity of the alpha and beta subunits of the yeast type I protein geranylgeranyltransferase was explored using prokaryotic expression of candidate genes. The Saccharomyces cerevisiae CDC43 and RAM2 genes were expressed in Escherichia coli and cell extracts examined for the ability to transfer [3H]geranylgeranyl diphosphate to an appropriate CaaX protein substrate. I...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m403469200